Department of Plant Pathology and Microbiology
Ping-Hu Wu
Ph.D. Student
Jian-Qun Huang
M.S. Student
| Year | Article |
|---|---|
| 2021 |
Potential adaptive genetic variation in Macrophomina phaseolina as revealed by environmental association on the whole-genome data Phytopathology [Abstract Collection] vol.111,page.S2.4. |
| 2021 |
Assessments of fungicide sensitivity and soybean resistance to red crown rot fungus Calonectria ilicicola Journal of Plant Medicine [Abstract Collection] vol.63,page.61 |
| 2021 |
Integration of RNA-Seq and reactive oxygen species inhibition assay to study microsclerotia development of Macrophomina phaseolina Journal of Plant Medicine [Abstract Collection] vol.63,page.57 |
| 2021 |
Sudden Death Syndrome of Soybean Journal of Plant Medicine [Abstract Collection] vol.63,page.39 |
| 2020 |
The diseases of adzuki bean seedling stem and root Journal of Plant Medicine [Abstract Collection] vol.62,page.72 |
| 2018 |
Characterization of a sudden death syndrome (SDS) core effector using comparative genomics between SDS-causing and non-SDS-causing Fusarium species Phytopathology [Abstract Collection] vol.108,page.110 |
| 2018 |
Temperature adaptation and fungicide sensitivity in Macrophomina phaseolina, the causal agent of charcoal rot on soybean and dry bean Phytopathology [Abstract Collection] vol.108,page.125 |
| 2018 |
Characterization of a novel transcription factor from Sclerotinia sclerotiorum induced during infection of pea Phytopathology [Abstract Collection] vol.108,page.42 |
| 2017 |
Meta-analysis for quantitative responses of soybean sudden death syndrome Phytopathology [Abstract Collection] vol.107,page.(Suppl.5): S5.168 |
| 2016 |
XVII Congress on Molecular Plant-Microbe Interactions Meeting Report Molecular Plant-Microbe Interactions vol.29,page.S6, S21 |
| 2015 |
Identification of a toxic protein, FvTox6, produced by Fusarium virguliforme that causes foliar symptoms typical of soybean sudden death syndrome Phytopathology [Abstract Collection] vol.105,page.(Suppl. 4) S4.27 |
| 2014 |
Secretory plant cell wall degrading enzyme expression analysis and polygalacturonase modeling of Fusarium virguliforme Phytopathology [Abstract Collection] vol.104,page.(Suppl. 3): S3.24 |
| Year | Article |
|---|---|
| 2016 |
Identification of phytotoxins produced by Fusarium virguliforme associated with foliar symptoms of sudden death syndrome and genome-wide association studies for soybean disease resistance Ph.D. Dissertation University of Illinois at Urbana-Champaign |
| 2016 |
Successful technologies and approaches used to develop and manage resistance against crop diseases and pests Emerging technologies for promoting food Security. Woodhead Publishing. page.43-66 |
Soybean (Glycine max (L.) Merr.) is one of the important crops in Taiwan. The exporting value of edamame (vegetable soybean) has reached to 8 billion USD annually in recent years, and edamame has been therefore regarded as "green gold" of Taiwan. Resistance breeding is a common goal for grain soybean and edamame, and seedling diseases such as seed decay and damping off occur frequently in the early growing stages. Management for seedling diseases relies on seed coating with pesticides. With the fact that at least five groups of pathogens can cause seedling diseases (Fusarium root rot, Macrophomina root rot, Phytophthora root rot, Pythium root rot, Rhizoctonia root rot) with risks of pesticide resistance, resistance breeding becomes a sustainable option for disease management.
Our lab studies edamame diseases in Taiwan, including isolation of endemic pathogens, screening for resistance, and applying genotyping-by-sequencing, QTL mapping, or genome-wide association study to identify molecular markers and resistance loci to provide a comprehensive academic and applied knowledge foundation for edamame diseases in Taiwan.
菌核菌(Sclerotinia sclerotiorum)、炭腐病菌(Macrophomina phaseolina)、立枯絲核菌(Rhizoctonia solani)等植物病原真菌可以形成菌核或微菌核等特化構造，在田間殘存並成為未來的感染源。因此研究菌核的形成機制，達到降低菌核於田間殘存的目標，是我們實驗室的研究興趣，並期望能針對形成菌核的病原真菌，開發整合性病害防治策略。
炭腐病菌之微菌核形成機制研究
碩士班劉軒豪同學以炭腐病菌的微菌核為研究材料，建立了四個菌核形成階段的組織萃取方式，並利用高通量定序及轉錄體方式，發現炭腐病菌的微菌核形成與活性氧(Reactive oxygen species, ROS)相關基因表現有顯著相關。後續在抗氧化劑與氧化刺激物的外源添加培養實驗中，深入發現O2-扮演了刺激菌絲分化成微菌核的重要關鍵角色。相關研究成果已發表在美國微生物學會的Microbiology Spectrum期刊。
立枯絲核菌AG-7之基因體與菌核遺傳研究
碩士班林鈺晟同學以立枯絲核菌AG-7為研究材料，以Oxford NanoPore及Illumina Sequencing完成了全球第一個AG-7菌絲融合群的基因體；另應用測序基因分型(GBS)鑑定154株分離自台灣的立枯絲核菌族群，並以全基因體關聯分析(GWAS)定位與菌核數量及菌核大小連鎖的SNPs。分析結果顯示立枯絲核菌的菌核形成能力應為可遺傳的性狀，其中與菌核數量連鎖的基因體區間有顯著富集的活性氧(ROS)相關基因功能。相關研究成果已發表在英國微生物學會的Microbial Genomics期刊。
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Dr. Chang welcomes one Postdoc researcher starting in July 2023. Please feel free to email contact for the position.
Dr. Chang welcomes one undergraduate student to take the "Direct Research" course in July 2023 (112-1 term), please feel free for email contact and stay in touch.
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Lab Alumni:
Hsien-Hao Liu (2021) Master Thesis: Integration of RNA-Seq and Reactive Oxygen Species Inhibition Assay to Study Microsclerotia Development of Macrophomina phaseolina.
Yu-Cheng Lin (2022) Master Thesis: The Disease and Sclerotia-forming Capability of Soybean Pathogen Rhizoctonia solani in Taiwan.